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Elevated levels of authentic plasma hydroperoxides in NIDDM
J Nourooz-Zadeh1, J Tajaddini-Sarmadi, S McCarthy
1Department of Medicine, University College London Medical School, U.K.
Diabetes
|September 1, 1995
Summary
Individuals with non-insulin-dependent diabetes mellitus (NIDDM) exhibit elevated plasma lipid hydroperoxides (ROOHs), indicating oxidative stress. This finding suggests NIDDM-associated oxidative stress may be independent of typical metabolic markers.
Area of Science:
- Biochemistry
- Endocrinology
- Oxidative Stress Research
Background:
- Non-insulin-dependent diabetes mellitus (NIDDM) is a complex metabolic disorder.
- Oxidative stress is implicated in diabetic complications, but specific markers require precise measurement.
Purpose of the Study:
- To accurately quantify plasma lipid hydroperoxides (ROOHs) in individuals with NIDDM.
- To investigate the association between NIDDM and elevated ROOH levels, exploring potential links to oxidative stress.
Main Methods:
- Utilized a precise ferrous oxidation with xylenol orange assay coupled with triphenylphosphine to measure authentic plasma ROOHs.
- Compared ROOH concentrations in 22 NIDDM patients against healthy control subjects.
- Measured plasma malondialdehyde (TBARM) and vitamin E levels as secondary markers.
Main Results:
- NIDDM patients showed significantly higher plasma ROOH levels (9.04 ± 4.3 μmol/l) compared to controls (3.76 ± 2.48 μmol/l; P < 0.0005).
- No significant difference in TBARM levels was observed between NIDDM and control groups.
- A trend towards lower vitamin E levels in NIDDM patients did not reach statistical significance.
Conclusions:
- Authentic ROOH measurement reveals a strong association between NIDDM and heightened oxidative stress.
- This oxidative stress in NIDDM may occur independently of lipid metabolism abnormalities or glycemic control.
- Further research is warranted to elucidate the mechanisms and implications of NIDDM-related oxidative stress.