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Related Experiment Videos

Does zona pellucida thickness influence the fertilization rate?

E Bertrand1, M Van den Bergh, Y Englert

  • 1Department of Obstetrics and Gynecology, Free University of Brussels, Erasme Hospital, Belgium.

Human Reproduction (Oxford, England)
|May 1, 1995
PubMed
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Zona pellucida thickness, a key factor in in-vitro fertilization (IVF), influences sperm penetration. Thinner zona pellucida in fertilized eggs suggests it impacts fertilization success in IVF programs.

Area of Science:

  • Reproductive biology
  • In-vitro fertilization (IVF)

Background:

  • The cumulus oophorus is routinely removed during human IVF to assess fertilization.
  • Zona pellucida thickness is measurable after cumulus removal and may affect fertilization outcomes.

Purpose of the Study:

  • To measure zona pellucida thickness in human oocytes.
  • To evaluate the influence of zona pellucida thickness on fertilization rates in IVF programs.

Main Methods:

  • Measurement of zona pellucida thickness in human oocytes during IVF.
  • Statistical analysis (one-way ANOVA) to compare thickness between fertilized and unfertilized oocytes.
  • Assessment of zona pellucida thickness changes over time and relation to ooplasm diameter.

Main Results:

Related Experiment Videos

  • Zona pellucida thickness ranged from 10 to 31 microns (mean 17.5 microns).
  • Fertilized oocytes had significantly thinner zona pellucida (16.6 ± 3.2 microns) compared to unfertilized oocytes (18.9 ± 4.0 microns; P < 0.001).
  • No significant change in zona pellucida thickness was observed between ovulation and 16-20 hours post-fertilization; thickness was unrelated to ooplasm diameter.

Conclusions:

  • Zona pellucida thickness is a significant factor influencing sperm penetration and fertilization rates in IVF.
  • A thick zona pellucida (≥ 22 microns) may indicate the need for assisted reproductive technologies like micro-injection.