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Pig plasma phospholipid transfer protein facilitates HDL interconversion
P Pussinen1, M Jauhianinen, J Metso
1National Public Health Institute, Department of Biochemistry, Helsinki, Finland.
Journal of Lipid Research
|May 1, 1995
Summary
Purified pig phospholipid transfer protein (PLTP) converts high-density lipoprotein (HDL) into distinct large and small particles. This conversion process involves the release of apolipoprotein A-I (apoA-I).
Area of Science:
- Lipid metabolism
- Protein biochemistry
- Cardiovascular research
Background:
- Phospholipid transfer protein (PLTP) plays a role in lipoprotein metabolism.
- Understanding PLTP's function in high-density lipoprotein (HDL) remodeling is crucial for cardiovascular health.
Purpose of the Study:
- To purify and characterize pig plasma PLTP.
- To investigate the effect of purified pig PLTP on HDL particle size distribution.
Main Methods:
- Ultracentrifugation, hydrophobic-, heparin-Sepharose-, and anti-pig-PLTP-affinity chromatography for PLTP purification.
- SDS-PAGE and gel filtration for molecular weight determination.
- Incubation of HDL with PLTP, followed by ultracentrifugation, native gradient gel electrophoresis, and high-resolution gel filtration to analyze particle size changes.
Main Results:
- Purified pig PLTP has a molecular weight of 78,000 Da.
- Incubation of human HDL3 and pig HDL with PLTP resulted in the formation of distinct large (10.8-11.5 nm) and small (7.6-7.9 nm) HDL particles.
- The conversion process was dependent on PLTP activity and time, and involved the release of apolipoprotein A-I.
Conclusions:
- Purified pig PLTP acts as a conversion factor for HDL particles.
- PLTP induces significant changes in HDL size distribution, generating distinct populations of smaller and larger particles.
- The release of apoA-I is integral to PLTP-mediated HDL conversion.