Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Description of an in vitro platelet function analyzer--PFA-100

S K Kundu1, E J Heilmann, R Sio

  • 1Dade International Inc., Miami, Florida, U.S.A.

Seminars in Thrombosis and Hemostasis
|January 1, 1995
PubMed
Summary

The new PFA-100 system offers a quantitative in vitro assessment of platelet function. It accurately detects platelet dysfunction by measuring closure time in whole blood samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of one or two successive annual applications of organic fertilisers on the enzyme activity of a soil under barley cultivation.

Bioresource technology·2001
Same author

Ketoconazole-induced conformational changes in the active site of cytochrome P450eryF.

Journal of molecular biology·2001
Same author

Comparative in vitro bacteriostatic and bactericidal activity of trovafloxacin, levofloxacin and moxifloxacin against clinical and environmental isolates of Legionella spp.

International journal of antimicrobial agents·2001
Same author

Serum leptin levels in cattle with different nutritional conditions.

Nutrition research (New York, N.Y.)·2001
Same author

A web page to teach neurology and neuropathology to medical students.

Neuropathology and applied neurobiology·2001
Same author

Constituents of Coriaria ruscifolia fruits.

Fitoterapia·2001

Area of Science:

  • Hematology
  • Clinical Diagnostics

Background:

  • Platelet dysfunction can lead to bleeding disorders.
  • Accurate and rapid diagnostic tools for platelet function are needed.

Purpose of the Study:

  • To introduce and evaluate the PFA-100 system for in vitro platelet function testing.
  • To assess the system's sensitivity to known platelet function impairments.

Main Methods:

  • The PFA-100 system utilizes a disposable cartridge with a collagen/epinephrine or collagen/adenosine diphosphate-coated membrane.
  • Blood is aspirated under vacuum, generating high shear rates that promote platelet activation and aggregation.
  • The time to occlude the aperture, termed 'closure time,' is measured.

Main Results:

Related Experiment Videos

  • Impairment of von Willebrand factor or inhibition of glycoprotein Ib/IIa receptors resulted in abnormal closure times.
  • The PFA-100 system demonstrated sensitivity to platelet adherence and aggregation abnormalities.
  • An antifibrinogen antibody did not affect closure times, indicating specificity.

    • Conclusions:

    • The PFA-100 system provides a quantitative and sensitive measure of platelet function in vitro.
    • Its accuracy, ease of use, and rapid results suggest potential for routine clinical evaluation of platelet disorders.