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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

The measurement of deoxynucleotide (dNTP) pools by radioimmunoassay (RIA)

W Aherne1, A Hardcastle, L Kelland

  • 1CRC Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK.

Advances in Experimental Medicine and Biology
|January 1, 1994
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...