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Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid
1United Graduate School of Agricultural Sciences, Kagoshima University, Japan.
Abstract:
An high-performance liquid chromatographic method with post-column derivatization has been developed for the simultaneous determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in fish tissues. Extracted AA and DHAA were separated by a Shim-pack SCR-1O1H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300 nm. The detection limits for both AA and DHAA were 0.1 microgram/ml.