Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid...

Area of Science:

• Analytical Chemistry
• Food Science
• Biochemistry

Background:

• Ascorbic acid (AA) and dehydroascorbic acid (DHAA) are vital antioxidants in biological tissues.
• Accurate quantification of AA and DHAA in fish is important for nutritional and quality assessments.
• Existing methods may lack the sensitivity or specificity required for complex fish matrices.

Purpose of the Study:

• To develop and validate a high-performance liquid chromatographic (HPLC) method for the simultaneous determination of AA and DHAA.
• To establish a sensitive and reliable analytical technique for fish tissue samples.
• To provide a robust method for assessing antioxidant status in fish.

Main Methods:

• High-performance liquid chromatography (HPLC) with post-column derivatization.
• Separation using a Shim-pack SCR-1O1H column.
• Detection via reaction with sodium hydroxide/sodium borohydride and UV monitoring at 300 nm.

Main Results:

• The developed HPLC method successfully separated and quantified both AA and DHAA simultaneously.
• Achieved low detection limits of 0.1 microgram/ml for both ascorbic acid and dehydroascorbic acid.
• The method demonstrated high performance for analyzing fish tissues.

Conclusions:

• The established HPLC method is suitable for the accurate and sensitive determination of AA and DHAA in fish.
• This technique provides a valuable tool for researchers studying fish nutrition and oxidative stress.
• The method's sensitivity and efficiency make it applicable for routine analysis.