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Histochemical and immunohistochemical techniques on acrylate embedded bone biopsies
Blut
|March 1, 1976
Summary
This study presents a new routine method for methacrylate-embedded bone biopsies, enabling histochemical staining for diagnosing hemoblastic disorders. The method preserves enzyme activity and improves diagnostic accuracy for conditions like multiple myeloma.
Area of Science:
- Histopathology
- Hematopathology
- Biomedical Diagnostics
Background:
- Methacrylate embedding of bone biopsies traditionally limits histochemical analysis.
- Previous methods restricted the application of sensitive staining procedures on semithin sections.
Purpose of the Study:
- To describe a routine method for methacrylate-embedded bone biopsies that retains enzyme activity and allows for sensitive staining.
- To enhance the diagnostic capabilities for hemoblastic disorders and plasma cell neoplasms.
Main Methods:
- Bone biopsies were fixed using 4% purified formaldehyde buffered in 0.1M sodium cacodylate.
- Dehydration was performed using a water-miscible glycolmethacrylate.
- The method allows for naphthol-AS-D-chloroacetate esterase, acid phosphatase, and immunoglobulin detection.
Main Results:
- The routine method successfully retains enzyme activity and allows for good sectioning.
- Naphthol-AS-D-chloroacetate esterase activity is observable in the neutropil cell lineage.
- Acid phosphatase activity and immunoglobulins can be demonstrated in atypical plasma cells of multiple myeloma.
Conclusions:
- This improved method expands the diagnostic utility of methacrylate-embedded bone biopsies.
- The technique offers reduced processing time and preserves enzyme activity in mailed samples.
- Enhanced histochemical analysis aids in the accurate diagnosis of hemoblastic disorders and multiple myeloma.