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Related Experiment Videos

Factor V turnover in a primate model

M D Rand1, S R Hanson, K G Mann

  • 1Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405-0068, USA.

Blood
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

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Researchers isolated and characterized baboon plasma factor V (FV) to model FV/FVa physiology in nonhuman primates. Baboon FV shares high sequence identity with human FV and exhibits similar thrombin activation pathways and clearance kinetics in vivo.

Area of Science:

  • Hematology
  • Biochemistry
  • Primate Physiology

Background:

  • Factor V (FV) is a crucial protein in the blood coagulation cascade.
  • Understanding FV/FVa physiology is vital for hemostasis research.
  • Nonhuman primate models offer valuable insights into human physiology.

Purpose of the Study:

  • To isolate and characterize baboon plasma factor V (FV).
  • To develop an in vivo model for studying FV/FVa physiology in nonhuman primates.
  • To compare baboon FV characteristics with human and bovine FV.

Main Methods:

  • Immunoaffinity chromatography using an anti-human FV monoclonal antibody for purification.
  • Thrombin-mediated activation and proteolytic pathway analysis.
  • In vivo studies involving infusion of radiolabeled baboon FV and its activation products into baboons, followed by radioactivity measurements and autoradiography.

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Main Results:

  • Purified baboon FV exhibited a specific activity of 1,940 U/mg.
  • Baboon FV activation by thrombin followed pathways similar to human and bovine FV.
  • Amino acid sequencing revealed 95% identity with human FV and 79% with bovine FV.
  • In vivo, intact baboon FV had a half-life (t1/2) of approximately 13 hours.
  • Thrombin-activated FV fragments (heavy and light chains) were cleared rapidly (t1/2 < 20 minutes), while intermediate fragments (220,000 and 150,000 Da) were cleared more slowly (t1/2 > 6 and > 30 hours, respectively).

Conclusions:

  • Baboon plasma FV has been successfully isolated and characterized.
  • The established baboon model allows for in vivo investigation of FV/FVa physiology.
  • Baboon FV demonstrates significant homology to human FV, supporting its use in preclinical studies.
  • The clearance kinetics of baboon FV and its activated fragments provide insights into FV metabolism in primates.