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Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7
1Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Fukui, Japan.
Bioscience, Biotechnology, and Biochemistry
|July 1, 1995
Abstract:
The gene encoding creatinine amidohydrolase (EC 3.5.2.10) was isolated from Pseudomonas sp. PS-7. The primary structure of creatinine amidohydrolase deduced from the nucleotide sequence showed that the protein is composed of 259 amino acids and has a molecular weight of 28,437. The enzymatic property of creatinine amidohydrolase produced by recombinant E. coli was identical with those by Pseudomonas sp. PS-7.