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Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography
C Labriola1, M Sousa, J J Cazzulo
1Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CONICET, Argentina.
Biological Research
|January 1, 1993
Abstract:
The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity.