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Related Experiment Videos

Multiple mechanisms regulate sympathetic neuronal phenotype

A K Hall1, S E MacPhedran

  • 1Department of Neuroscience, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.

Development (Cambridge, England)
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

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Sympathetic neurons in rats express neuropeptide Y (NPY), but regulation is unclear. Both intrinsic lineage and environmental factors influence NPY expression in developing neurons.

Area of Science:

  • Neuroscience
  • Developmental Biology

Background:

  • Adult rat sympathetic neurons utilize neuropeptides as cotransmitters with norepinephrine.
  • The regulatory factors governing neuropeptide expression in sympathetic neurons are largely unknown.
  • Approximately 60% of adult rat superior cervical ganglion (SCG) neurons express neuropeptide Y (NPY) in vivo.

Purpose of the Study:

  • To investigate whether the restricted expression of NPY in adult sympathetic neurons is an intrinsic property of the ganglia.
  • To determine the role of lineage commitment and environmental cues in regulating NPY expression during neuronal development.

Main Methods:

  • Cultured embryonic rat SCG neurons (E14.5) were examined for NPY immunoreactivity (-IR) after one week in vitro.
  • SCG precursors were labeled with a replication-incompetent retrovirus carrying lacZ to trace neuronal clones.

Related Experiment Videos

  • NPY expression within lacZ-labeled neuronal clones was analyzed after one week in culture.

    • Main Results:

    • 60% of cultured neurons derived from E14.5 rat SCG expressed NPY-IR, indicating intrinsic regulation of expression and developmental timing.
    • Two-thirds of neuronal clones were uniformly NPY-IR, suggesting lineage commitment to an NPY fate.
    • One-third of neuronal clones were mixed, containing both NPY-expressing and NPY-negative neurons, indicating environmental influences.

    Conclusions:

    • Developing sympathetic ganglia can intrinsically regulate NPY expression, including the number of neurons expressing NPY and the developmental timing.
    • Both neuroblast lineage commitment and extrinsic environmental cues play a role in determining the neuropeptide phenotype of sympathetic neurons.