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Specific direct fluorescent antibody identification of Mycobacterium leprae
Abstract:
In vitro cultivation of M. leprae requires a rapid, specific identification procedure for monitoring the cultures. A method utilizing direct FITC-coupled lepromatous, specific serum globulin is described in detail with suggestions for improvement. After various purification and adsorption procedures, notably against human liver powder and M. tuberculosis, a fluorescent serum preparation is obtained which specifically reacts with M. leprae and not with other mycobacteria.