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Cell swelling and depolarization in hemorrhagic shock
B D Borchelt1, P A Wright, J A Evans
1Department of Surgery, University of Maryland Hospital, Baltimore 21202, USA.
The Journal of Trauma
|August 1, 1995
Summary
A novel protein, circulating shock protein (CSP) 70, found in hemorrhagic shock increases intracellular water volume in various cells. This finding explains fluid shifts and challenges in restoring blood volume post-hemorrhage.
Area of Science:
- Physiology
- Cell Biology
- Biochemistry
Background:
- Intracellular water volume (IWV) changes in hemorrhagic shock were previously inferred but not directly measured.
- A circulating shock protein (CSP) 70 has been identified in hemorrhagic shock, known to depolarize cells.
Purpose of the Study:
- To directly measure the effect of CSP 70 on IWV.
- To investigate if CSP 70-induced cellular depolarization leads to increased intracellular water.
Main Methods:
- Cells were incubated with CSP 70.
- IWV was measured using the difference between 3H water and 14C-mannitol spaces.
Main Results:
- CSP 70 significantly increased IWV in rat red blood cells (9%) and H9c2 cells (22%).
- CSP 70 also increased IWV in dog red blood cells (11%) and white blood cells (31%).
Conclusions:
- Circulating CSP 70 depolarizes cells and increases intracellular water volume.
- This cellular fluid shift may contribute to the difficulty in restoring blood volume after severe hemorrhage.