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Related Experiment Videos

Circadian responses to light: the calmodulin connection

D A Golombek1, M R Ralph

  • 1Department of Psychology, University of Toronto, Ontario, Canada.

Neuroscience Letters
|June 9, 1995
PubMed
Summary

Calcium/calmodulin-dependent protein kinase II (CaM kinase II) and CREB are involved in the circadian rhythm

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Area of Science:

  • Neuroscience
  • Chronobiology
  • Molecular Biology

Background:

  • The suprachiasmatic nucleus (SCN) is the master circadian clock in mammals.
  • Light is a primary environmental cue that synchronizes circadian rhythms.
  • Signal transduction pathways mediate the effects of light on the SCN.

Purpose of the Study:

  • To investigate the role of CaM kinase II and CREB in mediating light-induced circadian responses.
  • To determine if CaM kinase II activity is necessary for photic entrainment of circadian rhythms.

Main Methods:

  • Administration of KN-62, a CaM kinase II inhibitor.
  • Administration of W-7, a calmodulin inhibitor.
  • Assessment of light-induced phase shifts in circadian rhythms.
  • Measurement of CREB phosphorylation in the SCN.

Main Results:

  • KN-62 attenuated light-induced phase shifts and reduced CREB phosphorylation.
  • W-7 produced similar effects to KN-62.
  • Neither drug completely blocked photic responses, indicating the involvement of other pathways.

Conclusions:

  • CaM kinase II activity and CREB are partially involved in mediating circadian responses to light.
  • Other signal transduction pathways also contribute to photic entrainment of circadian rhythms.

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