[Distribution of S-100 protein positive cells in conjunctiva]

We compared the distribution of S-100 protein positive cells in normal conjunctiva and in an induced allergic conjunctivitis model using Hartley strain guinea pigs. The animals were sensitized by intraperitoneal injection of ovoalbumin solution with hydroxy aluminum, followed by topical application of the ovoalbumin solution. The primary antibody was antibovine brain S-100 positive antibody, detected by the avidin biotin complex method. S-100 positive cells were present at the limbal corneal epithelium and on the skin of the palpebrae in both the normal group and the allergic conjunctivitis group, indicating the presence of Langerhans cells. In the allergic group, positive cells were also detected in palpebral conjunctiva and the germinal centers of conjunctival follicles. These cells were identified as follicular dendritic cells or interdigitating cells. In conclusion antigen presenting cells were present in the limbal conjunctiva, follicular and parafollicular area of conjunctival follicles, and palpebral skin in the allergic conjunctivitis models.