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Characterization of the O-polysaccharide of Pasteurella haemolytica serotype A1
1Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Carbohydrate Research
|February 24, 1993
Abstract:
Lipopolysaccharide was isolated from Pasteurella haemolytica biotype A, serotype 1 by using the phenol-water extraction procedure. Hydrolysis with mild acid afforded a high-molecular-weight antigenic O-chain. On the basis of 1D and 2D NMR spectral studies and microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a trisaccharide repeating unit having the structure -->3)-beta-D-Galp-(1-->3)-beta-D-GalpNAc-(1-->4)-beta-D-Galp-(1--> This O-polysaccharide antigen is expressed by several P. haemolytica biotype A serotypes.