Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proline isomerases function during heat shock

K Sykes1, M J Gething, J Sambrook

  • 1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050.

Proceedings of the National Academy of Sciences of the United States of America
|June 15, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chester treadmill police tests as alternatives to 15-m shuttle running.

Occupational medicine (Oxford, England)·2019
Same author

NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease.

Mucosal immunology·2017
Same author

Bioimpedance spectroscopy measurements of phase angle and height for age are predictive of outcome in children following surgery for congenital heart disease.

Clinical nutrition (Edinburgh, Scotland)·2017
Same author

Comparison of the effect of two doses of omeprazole on the squamous gastric mucosa in thoroughbred racehorses.

The Veterinary record·2014
Same author

The impact on utilities of differences in body weight among Canadian patients with type 2 diabetes.

Current medical research and opinion·2014
Same author

Negative prognostic factors for head and neck cancer in the young.

Journal of B.U.ON. : official journal of the Balkan Union of Oncology·2013
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Heat shock increases levels of cyclophilin (CYP) mRNA in yeast, with CYP1 and CYP2 playing roles in cellular survival during heat stress. FKBP gene expression was unaffected by heat.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Cyclophilins (CYPs) and FK506 binding proteins (FKBPs) are proline isomerases crucial for protein folding.
  • These enzymes are targets for immunosuppressive drugs.
  • Peptidyl-prolyl bond isomerization can limit protein folding rates.

Purpose of the Study:

  • To investigate the role of cyclophilins and FKBP in the heat shock response of Saccharomyces cerevisiae.
  • To determine if CYP and FKBP gene expression is affected by heat stress.
  • To assess the necessity of these genes for cellular survival under thermal stress.

Main Methods:

  • Quantitative analysis of mRNA levels in response to heat shock.
  • Reporter gene assays to study promoter activity.

Related Experiment Videos

  • Gene disruption (knockout) studies to assess functional roles in stress survival.

    • Main Results:

    • Heat shock induced a 2- to 3-fold increase in CYP1 mRNA levels.
    • CYP1 promoter contains heat shock response elements, conferring heat-inducible expression.
    • Disruption of CYP1 and CYP2 genes reduced yeast survival after heat exposure.
    • Expression of the FKBP gene (FKB1) was not heat-responsive and not essential for heat survival.

    Conclusions:

    • CYP1 and CYP2 are involved in the yeast heat stress response.
    • Heat shock upregulates the expression of specific cyclophilin genes.
    • Proline isomerases like cyclophilins play a significant role in cellular adaptation to thermal stress.