Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolated rat glomerular cells demonstrate L-type Ca(2+)-channel activity

G F McDermott1, R D Hurst, C I Whiteside

  • 1Department of Medicine, University of Toronto, Ontario, Canada.

Cell Calcium
|May 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: The effect of New Zealand blackcurrant on sport performance and related biomarkers: a systematic review and meta-analysis.

Journal of the International Society of Sports Nutrition·2021
Same author

The effect of New Zealand blackcurrant on sport performance and related biomarkers: a systematic review and meta-analysis.

Journal of the International Society of Sports Nutrition·2020
Same author

The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins.

Meat science·2014
Same author

Mucosal permeability testing: response.

Neurogastroenterology and motility·2013
Same author

The effect of aspirin and smoking on urinary excretion profiles of lactulose and mannitol in young women: toward a dynamic, aspirin augmented, test of gut mucosal permeability.

Neurogastroenterology and motility·2012
Same author

Post-mortem metmyoglobin reduction in fresh venison.

Meat science·2011

L-type calcium channels are present in rat glomerular and mesangial cells. These channels are activated by depolarization and agonists, and their function may be hormone-specific.

Area of Science:

  • Nephrology
  • Cell Physiology
  • Pharmacology

Background:

  • L-type calcium channels play critical roles in cellular function.
  • Their presence and specific roles in glomerular cells require detailed investigation.

Purpose of the Study:

  • To investigate the presence and function of L-type calcium channels in rat glomerular cells.
  • To determine the effects of calcium channel antagonists on these cells.

Main Methods:

  • Isolated rat glomeruli were used to measure 45Ca2+ uptake and efflux.
  • Cultured rat mesangial cells were analyzed for cytosolic Ca2+ using microfluorometry.
  • Cells were stimulated with KCl, Bay K 8644, and gramicidin, with or without antagonists like nifedipine and verapamil.

Related Experiment Videos

Main Results:

  • KCl and Bay K 8644 stimulated 45Ca2+ uptake and efflux in isolated glomeruli, inhibited by nifedipine and verapamil.
  • Voltage-dependent calcium channel activity was confirmed by sequential stimulation.
  • In cultured mesangial cells, KCl, gramicidin, and Bay K 8644 increased Ca2+ influx, inhibited by verapamil.
  • Verapamil did not affect endothelin-stimulated Ca2+ signaling.

Conclusions:

  • L-type calcium channels are present on rat glomerular endothelial and/or mesangial cells in vivo.
  • These channels are also found on cultured rat mesangial cells.
  • The activation of these channels may exhibit hormone specificity.