Related Experiment Videos
[Preparation of a specific immunogen based on bacteriophage M13]
Molekuliarnaia Biologiia
|May 1, 1993
Summary
Researchers created a recombinant phage displaying a human immunodeficiency virus (HIV) peptide. Antibodies against this phage recognized HIV proteins, suggesting potential for vaccine development.
Area of Science:
- Molecular Biology
- Virology
- Immunology
Context:
- Bacteriophage M13 was engineered as an expression vector for displaying peptides on its surface.
- A recombinant phage was generated to display an antigenic determinant from the human immunodeficiency virus (HIV) gag protein p17.
Purpose:
- To assess the immunogenicity of a recombinant phage displaying an HIV peptide.
- To determine if antibodies generated against the fusion phage can recognize HIV viral proteins.
Summary:
- The recombinant phage was used as an immunogen in rabbits.
- ELISA confirmed that antibodies against the fusion phage reacted with both the 17-kDa core protein and the p55 polyprotein precursor of HIV.
Impact:
- These findings demonstrate the potential utility of this recombinant phage system in developing novel vaccines against HIV.
- The study validates the use of phage display technology for generating immunogens that elicit antibodies against specific viral targets.