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Insulin-like growth factor II in the mink (Mustela vison): determination of a cDNA nucleotide sequence and
T J Ekström1, B M Bäcklin, Y Lindqvist
1Centre for Biotechnology, NOVUM Research Park, Huddinge, Sweden.
Abstract:
Mink cDNA for insulin-like growth factor II (IGF-II) has been isolated by cDNA synthesis from bulk mRNA and subsequent PCR-screening. Positive clones were sequenced and analyzed. Analysis of a cDNA revealed that, compared with human, the mature mink IGF-II peptide contains 68 instead of 67 amino acids with a serine insertion at residue 40. With this exception, the homology between the human and mink mature peptide is 100% and is 94% between rat and mink. Comparing IGF-II transcripts in fetal and adult mink liver, fetal tissue contains three transcripts of 5.8, 4.5, and 4.0 kb. In contrast, adult liver expresses low levels of a 4.6-kb transcript.