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CDw75 antigen expression in breast lesions

W Reed1, B K Erikstein, S Funderud

  • 1Department of Pathology, Norwegian Radium Hospital, Oslo.

Pathology, Research and Practice
|May 1, 1993
PubMed
Summary
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This study investigated CDw75 expression in breast lesions. Elevated CDw75 was found in malignant breast carcinomas and some benign proliferating lesions, suggesting potential epithelial cell activation.

Area of Science:

  • Oncology
  • Glycobiology
  • Immunohistochemistry

Background:

  • Aberrant oligosaccharide structures on cell surface glycoproteins and glycolipids are implicated in tumorigenesis.
  • Sialyltransferases, enzymes that add sialic acid to glycoconjugates, are often elevated in tumors and patient serum.
  • CDw75 is an epitope recognized by the monoclonal antibody HH2, associated with beta-galactosyl alpha 2,6-sialyltransferase activity.

Purpose of the Study:

  • To examine the expression of the CDw75 epitope in various breast lesions.
  • To correlate CDw75 expression with tumor type, malignancy grade, and metastatic potential.

Main Methods:

  • Immunohistochemical analysis using the monoclonal antibody HH2 to detect the CDw75 epitope.
  • Evaluation of 142 breast tissue samples, including carcinomas in situ, invasive carcinomas, and benign lesions.

Related Experiment Videos

  • Assessment of correlation between CDw75 reactivity and clinicopathological features.

    • Main Results:

    • CDw75 expression was observed in 21% of carcinomas in situ and 35% of invasive carcinomas, with cytoplasmic and occasional membrane staining.
    • No correlation was found between CDw75 expression and metastatic potential in malignant lesions.
    • Positive CDw75 immunoreactivity was detected in proliferating benign lesions like papillomas and fibrocystic disease, but rarely in normal tissue, fibroadenomas, or phyllodes tumors.
    • CDw75 expression was more frequent in high-grade malignant carcinomas.

    Conclusions:

    • CDw75 expression is altered in breast cancer and can be present in benign proliferating lesions.
    • The presence of CDw75 in benign lesions may indicate epithelial cell activation.
    • CDw75 expression correlates with higher malignancy grade in breast carcinomas.