Surveillance of gram-negative bacteria for Pseudomonas aeruginosa lipoprotein I using a monoclonal antibody

We prepared an IgG1 murine monoclonal antibody, PS2, that recognized the 8-kDa outer membrane lipoprotein I of Pseudomonas aeruginosa. The biodot-immunoblot technique was used to test 470 bacterial isolates for their reactivity with MAb PS2. PS2 recognized 298 of 300 P. aeruginosa clinical isolates for a sensitivity of 99.3%. PS2 reacted with all Fisher-Devlin immunotype and International Antigenic Type P. aeruginosa strains. Forty-seven isolates of other Pseudomonas species were tested and only three P. cepacia reacted. PS2 did not react with 118 of 123 other gram-negative isolates, but reacted weakly with 3 of 17 Enterobacter aerogenes, 1 of 11 Eschericia coli and 1 of 2 Proteus vulgaris. The specificity of PS2 for P. aeruginosa was 95%.