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Purification of normal human bone-marrow-derived basophils
M Arock1, M D Mossalayi, L Le Goff
1Laboratoire d'Hématologie, Faculté de Pharmacie de Paris V, France.
International Archives of Allergy and Immunology
|January 1, 1993
Summary
Researchers developed a simple method to isolate pure, functional basophils from human hematopoietic cells. This technique yields large quantities of these important immune cells for further study.
Area of Science:
- Immunology
- Cell Biology
- Hematopoiesis
Background:
- Basophils and eosinophils share a common differentiation pathway.
- Factors regulating terminal commitment to basophil or eosinophil lineages are not fully defined.
- Interleukin-3 (IL-3) is a key differentiation factor for both cell types.
Purpose of the Study:
- To develop a rapid and simple method for isolating pure populations of normal human basophils.
- To obtain functional basophils for further research.
Main Methods:
- Human hematopoietic cells were cultured with recombinant IL-3 for 3-4 weeks.
- Cells were purified using gradient density centrifugation over a Percoll gradient.
- A subsequent adhesion step on tissue-culture-treated plastic was employed.
Main Results:
- A cell population of 94% pure normal basophils and precursors was reproducibly obtained.
- No mast cells were detected in the isolated population.
- Isolated basophils contained high histamine levels and released it upon stimulation, demonstrating functional capacity.
Conclusions:
- A simple and effective method for producing large quantities of pure, functional human basophils from bone marrow-derived hematopoietic cells was established.
- This method facilitates further investigation into basophil biology and function.