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[Optimizing parameters in photodynamic virus inactivation of fresh frozen plasma]
H Mohr1, B Lambrecht, H Schmitt
1DRK-Blutspendedienst Niedersachsen, Springe.
Abstract:
Fresh plasma from single-blood donations is virus-inactivated by illuminating the plasma units in their respective plastic bags with visible light for 1 h in the presence of 1 microM of the photoactive dye methylene blue. For "soft" viruses, e.g. the togavirus Semliki Forest, 0.3 microM are sufficient to achieve complete inactivation within 5-10 min. The infectious titer is reduced by 5-6 log 10 steps. To achieve the same degree of reduction in infectivity for more resistant viruses, e.g. vesicular stomatitis virus or SV 40, the virus-containing plasma at a dye concentration of 1 microM has to be light-treated for 30-45 min. More time is required at lower dye concentrations. These findings determined the conditions chosen for photodynamic virus inactivation of fresh plasma from single-blood donations.
Insights
Photodynamic virus inactivation using methylene blue dye and visible light effectively reduces infectious titers in fresh plasma. This method ensures pathogen safety in blood donations by inactivating various viruses.
Area of Science:
- Biotechnology
- Virology
- Blood Transfusion Safety
Context:
- Fresh plasma from single-blood donations requires pathogen inactivation to ensure transfusion safety.
- Photodynamic inactivation is a promising method for virus clearance in biological products.
Purpose:
- To determine the optimal conditions for photodynamic virus inactivation in fresh plasma using methylene blue and visible light.
- To assess the efficacy of this method against different types of viruses.
Summary:
- Fresh plasma was treated with visible light and methylene blue (0.3–1 µM).
- Complete inactivation of "soft" viruses like Semliki Forest virus was achieved in 5–10 minutes with 0.3 µM dye.
- More resistant viruses, such as vesicular stomatitis virus and SV 40, required 30–45 minutes of treatment with 1 µM dye for a 5–6 log10 reduction in infectious titer.
Impact:
- Establishes effective parameters for photodynamic virus inactivation in plasma, enhancing blood product safety.
- Demonstrates the potential of photodynamic methods for broad-spectrum virus clearance in clinical settings.
- Contributes to the development of safer transfusion practices by reducing the risk of viral transmission.