Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stopped lower buffer flow electrofractionation: simple electrofractionation for complex protein mixture

J Pyun1, H Choi, J Park

  • 1Department of Chemistry, College of Natural Science, Seoul National University, Korea.

Analytical Biochemistry
|November 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of organic loading rate on bioflocculant production derived from waste glycerin pitch and mixed microbial culture in single-step system.

Bioresource technology·2026
Same author

<i>Notum</i> Regulates Tooth Root Morphogenesis by Modulating Wnt Signaling.

Journal of dental research·2025
Same author

Dermoscopy for lipidized dermatofibroma: A useful diagnostic tool.

Annales de dermatologie et de venereologie·2024
Same author

Low household income increases the risk of tuberculosis recurrence: a retrospective nationwide cohort study in South Korea.

Public health·2023
Same author

Tuberculosis and risk of Parkinson's disease: A nationwide cohort study.

Pulmonology·2022
Same author

Search for Dark Matter Axions with CAST-CAPP.

Nature communications·2022
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

A novel stopped lower buffer flow electrofractionation (SFE) system overcomes electric resistance issues, enabling efficient purification of complex protein mixtures. This advancement expands the utility of electrofractionation for challenging protein separation applications.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Electrofractionation is a technique for protein separation, often modeled after liquid chromatography.
  • The standard system faced limitations in purifying complex protein mixtures due to restricted current flow caused by column holder resistance.

Purpose of the Study:

  • To develop an improved electrofractionation system for overcoming electric resistance limitations.
  • To enhance the purification of complex and low-concentration protein mixtures.

Main Methods:

  • A stopped lower buffer flow electrofractionation (SFE) system was designed, eliminating the need for a column holder.
  • Prestained markers were utilized for real-time detection of protein positions during the electrofractionation process.

Related Experiment Videos

Main Results:

  • The SFE system effectively addressed the electric current flow problem inherent in previous designs.
  • The technique demonstrated efficiency in the partial purification of a 32P-labeled protein from rat liver microsomes.

Conclusions:

  • The SFE system significantly broadens the applicability of electrofractionation for purifying challenging protein samples.
  • This method offers a viable solution for the separation of complex and low-abundance protein mixtures.