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Delayed type hypersensitivity responses in mice transplanted with rat hepatocytes
S Tanabe1, Y Taura, S Furusawa
1Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
The Journal of Veterinary Medical Science
|December 1, 1994
Summary
Investigating delayed type hypersensitivity (DTH) responses to rat hepatocytes in mice revealed that intravenous or intrasplenic immunization induces suppressive spleen cells. These cells significantly reduce DTH responses compared to subcutaneous immunization.
Area of Science:
- Immunology
- Transplantation Biology
Background:
- Delayed type hypersensitivity (DTH) is a critical immune response.
- Xenogeneic hepatocyte transplantation is a potential therapeutic strategy.
- Understanding immune responses to xenografts is vital for transplantation success.
Purpose of the Study:
- To investigate the impact of different transplantation routes on DTH responses to xenogeneic hepatocytes.
- To identify mechanisms of immune tolerance or suppression in xenotransplantation models.
Main Methods:
- Mice received subcutaneous (s.c.), intrasplenic (i.s.), or intravenous (i.v.) transplantation of rat hepatocytes.
- Delayed type hypersensitivity (DTH) responses were measured.
- Spleen cells from immunized mice were co-transferred to naive recipients to assess suppressive effects.
Main Results:
- Pre-immunization via i.s. or i.v. routes significantly lowered DTH responses compared to s.c. immunization.
- Co-transfer of spleen cells from i.v. immunized mice suppressed DTH responses.
- Spleen cells from i.s. immunized mice did not suppress DTH responses when co-transferred with s.c. immunized spleen cells.
Conclusions:
- Intravenous or intrasplenic immunization with xenogeneic hepatocytes induces antigen-specific suppressive spleen cells.
- These suppressive cells play a role in modulating DTH responses to rat hepatocytes.
- Findings suggest potential strategies for inducing tolerance in xenotransplantation.