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Binding proteins and membrane transport

D L Oxender, S Quay

    Annals of the New York Academy of Sciences
    |December 30, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Two active transport systems exist: membrane-bound and binding protein-dependent. Binding proteins enhance solute transport affinity by interacting with membrane components, utilizing phosphate bond energy.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Cell Biology

    Background:

    • Two distinct types of active transport systems have been identified.
    • These systems are crucial for cellular nutrient uptake and waste removal.

    Purpose of the Study:

    • To elucidate the distinct energy coupling mechanisms of membrane-bound and binding protein-dependent active transport systems.
    • To define the specific roles and characteristics of binding proteins in active transport.

    Main Methods:

    • Analysis of membrane vesicles to study membrane-bound systems.
    • Investigation of osmotic-shock-sensitive systems requiring binding proteins.
    • Characterization of energy sources (respiration, ATP-hydrolysis, glycolysis, oxidative phosphorylation) for each system.

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    Main Results:

    • Membrane-bound systems utilize cellular energy from membrane states derived from respiration or ATP-hydrolysis.
    • Binding protein systems are directly coupled to phosphate bond energy from glycolysis or oxidative phosphorylation.
    • Binding proteins, found in the periplasmic space, do not directly translocate solutes but possess a secondary binding site for membrane interaction.

    Conclusions:

    • Binding proteins significantly increase the affinity of the transport system for specific solutes.
    • This enhanced affinity is mediated through interaction with a membrane component, potentially acting as a substrate.
    • The two active transport systems exhibit distinct energy coupling and functional roles for their components.