[Detection of antinuclear autoantibodies by the indirect Coon's method]

A variant of the indirect method of fluorescent antibodies for revealing antinuclear autoantibodies and simultaneously for identifying the tissue- and organ-specific autoantibodies is suggested. As substrate, cryostatic sections of the rat's liver, heart, kidney which were fixed for 3 minutes in 96 degrees ethanol were used. Detection of autoantibodies is performed with the use of pure labeled autoantibodies to human IgG. The method described makes it possible to obtain easily compared and reproducible results.