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Related Experiment Videos

Do IgE-IgG complexes occur in the circulation?

L Boluda1, L Berrens

  • 1Research Group C.B.F. LETI, Tres Cantos, Madrid, Spain.

Clinical and Experimental Immunology
|April 1, 1995
PubMed
Summary
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Researchers investigated IgG anti-IgE autoantibodies in allergic individuals. They found these autoantibodies form in vitro on test plates, not pre-formed in circulation, challenging previous interpretations of enzyme immunoassays for allergy diagnostics.

Area of Science:

  • Immunology
  • Allergy Diagnostics

Background:

  • Immunoglobulin E (IgE) plays a central role in allergic reactions.
  • The presence and significance of IgG anti-IgE autoantibodies in allergic individuals require clarification.

Purpose of the Study:

  • To investigate the formation and presence of IgG anti-IgE autoantibodies in human allergic sera.
  • To determine the correlation between total IgE levels and IgG anti-IgE autoantibodies.
  • To elucidate the in vivo or in vitro origin of IgG anti-IgE autoantibody detection.

Main Methods:

  • Enzyme immunoassay using microplates coated with anti-human IgE monoclonal antibodies (MoAb).
  • Detection of total IgE using peroxidase-labelled anti-human IgE MoAb.
  • Evaluation of IgG anti-IgE autoantibodies using anti-IgG MoAb.

Related Experiment Videos

  • Molecular sieving on gel columns and ultrafiltration experiments.

    • Main Results:

    • No correlation was found between total IgE and IgG anti-IgE levels in allergic individuals' sera.
    • IgG anti-IgE complexes do not pre-form in circulation.
    • These complexes arise de novo on the anti-IgE-coated solid phase during in vitro incubation.

    Conclusions:

    • The detection of IgG anti-IgE autoantibodies in immunoassays is an in vitro phenomenon.
    • IgG anti-IgE autoantibodies likely react with IgE after conformational changes induced by assay conditions or allergens.
    • This finding necessitates a re-evaluation of diagnostic interpretations involving IgG anti-IgE in allergy.