Related Experiment Videos
Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa
L Benvenisti1, S Koby, A Rutman
1Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Gene
|March 21, 1995
Abstract:
A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene encoding the heat-shock sigma factor sigma 32 has been cloned and sequenced. The gene is expressed in Escherichia coli and complements an rpoH- strain. An open reading frame encoding 284 amino acids shows 61% identity and 78% similarity to the RpoH protein of Escherichia coli.