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Vital staining of coronal dentin in monkey teeth
Oral Surgery, Oral Medicine, and Oral Pathology
|April 1, 1978
Summary
Monkey incisor dentin exposed by wear stained distinctly with vital dyes. A central zone remained unstained due to blocked dentinal tubules in the pulp horn.
Area of Science:
- Dentistry
- Oral Biology
- Histology
Background:
- Tooth attrition can expose dentin to the oral environment.
- Vital staining is a technique used to assess tissue permeability and characteristics.
Purpose of the Study:
- To investigate the staining patterns of vital dyes in monkey incisor dentin exposed by attrition.
- To determine the reasons for differential staining in exposed dentin.
Main Methods:
- Monkey incisor teeth with attrition-exposed dentin were subjected to vital staining using various pH and redox indicator dyes.
- Dentin staining was examined macroscopically and microscopically.
Main Results:
- Coronal dentin stained distinctly with all introduced dyes.
- A narrow, centrally located unstained zone was observed, extending from the pulp horn to the incisal edge.
- This unstained zone correlated with an area where pulpal ends of dentinal tubules were blocked by atubular hard tissue.
Conclusions:
- The unstained zone in attrition-exposed incisor dentin is not due to oral environment exposure but rather to blocked dentinal tubules.
- Atubular hard tissue formation in the pulp horn region of incisors occludes dentinal tubules, preventing dye penetration.