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Microsatellite-based parentage control in cattle
M L Glowatzki-Mullis1, C Gaillard, G Wigger
1Institute for Animal Breeding, University of Berne, Switzerland.
Animal Genetics
|February 1, 1995
Abstract:
As a new approach to parentage control we developed two multiplex coamplification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite polymorphisms were visualized by automated fluorescence detection on the Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis software. Allele frequency data were determined from 238 animals. Thirty-five bovine parentage control cases not solvable by conventional blood typing could be solved.