No evidence of effect on male mice germ cells after acute treatment with thiram

Thiram is a dithiocarbamate compound widely used for industrial processes and agriculture. Animal studies reveal that this compound may affect the male reproductive system. Aim of this study was to test, using sensitive testicular parameters, whether thiram directly affects germinal cells. For this purpose, B6C3F1 mice were intraperitoneally injected with thiram in oil (single dose: 75 mg/kg; repeated five daily doses: 25 mg/kg). Although both treatments were toxic, none of the parameters examined, i.e., testis weight, spermatid head number, specific enzyme levels at different times after treatment (14, 28, 35, 56 days) showed significant variations from the controls. On the contrary, in the positive controls (treated with chlorambucil), a marked reduction of sperm head number as well as a decrease of lactate dehydrogenasex and sorbitol dehydrogenase activity levels were evidenced at day 28, with a tendency to recover at day 35. Under these conditions thiram did not cause cytotoxicity on differentiating spermatogonia and on late spermatocyte stages of mice gonads.