Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Semiautomated DNA probe mapping using digital imaging microscopy: II. System performance

M Sakamoto1, D Pinkel, L Mascio

  • 1Division of Molecular Cytometry, University of California, San Francisco 94143-0808, USA.

Cytometry
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mini-invasive Anterior Petrosal Intertentorial Approach for Trigeminal Neuralgia: Rationale and Limits of the Technique.

Journal of neurological surgery. Part B, Skull base·2026
Same author

CIMT 2023: report on the 20th Annual Meeting of the Association for Cancer Immunotherapy.

Immuno-oncology technology·2023
Same author

Comparison of disease severity in glaucoma patients identified by screening in the 1990s and in routine clinical care in the 2010s in Sweden.

Acta ophthalmologica·2023
Same author

Combined petrosal approach: a systematic review and meta-analysis of surgical complications.

Neurosurgical review·2023
Same author

Genomic loci associated with performance limiting equine overriding spinous processes (kissing spines).

Research in veterinary science·2022
Same author

The organisational response of a hospital critical care service to the COVID-19 pandemic: The Groote Schuur Hospital experience.

The Southern African journal of critical care : the official journal of the Critical Care Society·2022
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

This study evaluates a semiautomated system for mapping DNA probes on chromosomes using fluorescence in situ hybridization (FISH). The system accurately determines probe locations, aiding in gene mapping and understanding chromosome structure.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Accurate mapping of cloned probes along metaphase chromosomes is crucial for genetic research.
  • Existing mapping methods can be labor-intensive and may lack precision.

Purpose of the Study:

  • To evaluate a semiautomated, multicolor image-analysis system for mapping cloned probes.
  • To assess the system's accuracy and reliability in determining probe locations on chromosomes.

Main Methods:

  • Utilized fluorescence in situ hybridization (FISH) for probe localization.
  • Employed automatic acquisition of multicolor images of chromosomes and probes.
  • Automated determination of fractional probe locations relative to the short arm telomere (FLpter).

Related Experiment Videos

Main Results:

  • The system demonstrated high accuracy, with standard deviations of FLpter measurements averaging 3.4 Mb for phage and 2.6 Mb for cosmid probes.
  • Probe order could be determined with 95% confidence for separations greater than 2.5 Mb.
  • Converted FLpter values to band locations using digital imaging microscopy, outperforming ISCN ideogram conversions.

Conclusions:

  • The semiautomated system provides accurate and reliable probe mapping along metaphase chromosomes.
  • This technology enhances the precision of gene mapping and chromosome analysis.
  • Digital imaging microscopy offers a superior method for converting probe locations to band assignments.