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Phage conversion in Salmonella enterica serotype Enteritidis: implications for epidemiology
Epidemiology and Infection
|April 1, 1995
Summary
Researchers developed a phage conversion model for Salmonella Enteritidis, successfully altering phage types and revealing relationships within clonal lineages. This system aids in understanding bacterial typing and phage interactions.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Phage typing is crucial for Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) surveillance and epidemiology.
- Understanding phage-bacterial interactions is key to interpreting phage typing results and bacterial relationships.
Purpose of the Study:
- To establish a model system for studying phage conversion in Salmonella Enteritidis.
- To investigate the relationships between different phage types within a clonal lineage (SECLIII).
- To determine if Salmonella enterica serotype Typhi and Salmonella enterica serotype Paratyphi B possess type-determining phages.
Main Methods:
- Utilized temperate phages (1, 2, 3, and 6) from the standard phage typing scheme.
- Applied phages to convert existing Salmonella Enteritidis phage types into new ones.
- Examined phage lysis patterns and inter-type relationships within the SECLIII clonal lineage.
Main Results:
- Successfully converted various Salmonella Enteritidis phage types, including PT4 to PT8, PT6a to PT4/PT7, PT13 to PT13a, and PT15 to PT11.
- Identified novel phage lysis patterns.
- Demonstrated the conversion of PT1 to PT20 and PT15 to PT11, further validating the model.
Conclusions:
- The developed model system effectively demonstrates phage conversion in Salmonella Enteritidis.
- Phage conversion can alter established phage types, highlighting the dynamic nature of bacterial typing.
- The model provides insights into the relationships between phage types and the potential presence of type-determining phages in related Salmonella serotypes.