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A cytofluorometric method of counting trypanosomes

J N Mills, V E Valli

    Tropenmedizin Und Parasitologie
    |March 1, 1978
    PubMed
    Summary
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    A new automated method uses vital staining and cytofluorometry to count Trypanosoma congolese. This technique accurately quantifies trypanosomes in separated samples and whole bovine blood above 1,000 parasites/microliter.

    Area of Science:

    • Veterinary Parasitology
    • Biotechnology
    • Medical Diagnostics

    Background:

    • Accurate quantification of trypanosomes is crucial for diagnosing and managing trypanosomiasis.
    • Existing manual counting methods are time-consuming and labor-intensive.
    • Development of rapid, automated diagnostic tools is needed for effective disease control.

    Purpose of the Study:

    • To develop and validate a rapid, automated method for counting Trypanosoma congolese.
    • To assess the accuracy of the method in different sample types and concentrations.
    • To provide a more efficient alternative to manual trypanosome counting.

    Main Methods:

    • Vital staining of trypanosomes with acridine orange.
    • Utilizing cytofluorometric analysis for automated counting.

    Related Experiment Videos

  • Employing DEAE cellulose for separation of Trypanosoma congolese.
  • Testing the method on heparinized whole bovine blood.

    • Main Results:

    • The automated cytofluorometric method accurately counted Trypanosoma congolese after DEAE cellulose separation across all concentrations.
    • Accurate counts were achieved in whole bovine blood samples with parasite levels exceeding 1,000 per microliter.
    • Interference from small fluorescent particles in whole blood affected counts at lower parasite concentrations.

    Conclusions:

    • Cytofluorometric analysis offers a rapid and accurate automated method for counting Trypanosoma congolese.
    • The method shows promise for diagnosing trypanosomiasis, particularly in separated samples or high-parasitemia blood.
    • Further refinement may be needed to overcome interference issues in low-parasitemia whole blood samples.