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Related Experiment Videos

Platelet-activating factor mediates trinitrobenzene induced colitis

W E Longo1, G Polities, A M Vernava

  • 1Department of Surgery, St Louis University School of Medicine, Missouri 63110-0250.

Prostaglandins, Leukotrienes, and Essential Fatty Acids
|December 1, 1994
PubMed
Summary

Platelet-activating factor (PAF) plays a role in experimental colitis induced by trinitrobenzene sulfonic acid (TNB). Blocking PAF with WEB-2170 reduced inflammation, suggesting PAF is a key mediator in this condition.

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Area of Science:

  • Gastroenterology
  • Immunology
  • Pharmacology

Background:

  • Platelet-activating factor (PAF) is implicated in inflammatory conditions like colitis.
  • Eicosanoids are second messengers involved in PAF's inflammatory effects.
  • Experimental colitis models are crucial for understanding inflammatory bowel disease.

Purpose of the Study:

  • To investigate if trinitrobenzene sulfonic acid (TNB) induces PAF and eicosanoid release in rabbit colon.
  • To determine if a PAF antagonist can block TNB-induced PAF release and subsequent inflammation.
  • To elucidate the role of PAF in the pathogenesis of TNB-induced experimental colitis.

Main Methods:

  • Utilized an ex vivo isolated rabbit colon perfusion model.
  • Induced colitis via intracolonic administration of TNB in ethanol.

Related Experiment Videos

  • Measured tissue and perfusate levels of PAF and eicosanoids (PGE2, 6-keto-PGF1α, TXB2, LTB4) using ELISA.
  • Administered PAF receptor antagonist WEB-2170 before TNB challenge.

    • Main Results:

    • TNB administration significantly increased colonic tissue PAF levels compared to controls.
    • WEB-2170 pretreatment successfully blocked TNB-induced PAF release.
    • TNB stimulated luminal eicosanoid release (except LTB4) and suppressed tissue prostanoids.
    • WEB-2170 inhibited luminal eicosanoids and suppressed PGE2 and prostacyclin, but not TXB2 tissue levels.

    Conclusions:

    • Exogenous TNB stimulates PAF release in the rabbit colon, indicating PAF's role in TNB-induced colitis.
    • PAF receptor antagonism with WEB-2170 mitigates key inflammatory mediators.
    • These findings suggest PAF is a significant contributor to tissue injury in experimental colitis.