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Determination of lysine with ninhydrin-ferric reagent
C L Hsieh1, K P Hsiung, J C Su
1Union Chemical Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
Analytical Biochemistry
|January 1, 1995
Abstract:
Ninhydrin-ferric reagent reacts highly and specifically with lysine at a pH value of 1.0. Ferric ion inhibits the reaction of ninhydrin with proline, ornithine, glycine, arginine, and histidine. This method can be used to determine a lysine sample of high concentration without dilution. It has a linear response range from 0.0625 to 0.5 mg lysine.HCl.