Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Normal modes as refinement parameters for the F-actin model

M M Tirion1, D ben-Avraham, M Lorenz

  • 1Department of Physics, Clarkson University, Potsdam, New York 13699-5820.

Biophysical Journal
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whose Responsibility Is It? A Community-Level Situational Analysis of Oral Health Care in Amsterdam.

JDR clinical and translational research·2025
Same author

Situational Analysis of European and International Oral Health Policy Making for Quality Improvement.

JDR clinical and translational research·2025
Same author

Characteristics of children with severe preschool asthma prior to starting the TIPP study.

Frontiers in pediatrics·2025
Same author

Quality Improvement in General Dental Practice: Situational Analysis for the United Kingdom and Germany.

JDR clinical and translational research·2025
Same author

Deliberative Improvement of Oral Care Quality: The Horizon Europe DELIVER Project.

JDR clinical and translational research·2023
Same author

Simulation of Radiative Transfer Within X-ray Microcalorimeter Absorbers.

Journal of low temperature physics·2022
Same journal

Heterogeneous binding of SARS-CoV2 fusion peptide on complex cellular membranes enhances its fusogenicity.

Biophysical journal·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
See all related articles

Slow normal modes of globular actin (G-actin) were used to refine filamentous actin (F-actin) models. This method improved structural accuracy, revealing key changes in actin

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biophysics

Background:

  • Actin is a crucial protein for cell structure and motility.
  • Understanding filamentous actin (F-actin) structure is key to deciphering its biological functions.
  • Previous models of F-actin lacked atomic-level detail in certain regions.

Purpose of the Study:

  • To refine the structural model of F-actin using normal mode analysis.
  • To investigate the collective domain rearrangements in G-actin relevant to F-actin structure.
  • To improve the fit of F-actin models to X-ray fiber diffraction data.

Main Methods:

  • Utilized slow normal modes of globular actin (G-actin) as structural parameters.
  • Refined the F-actin model against 8-Å resolution X-ray fiber diffraction data.

Related Experiment Videos

  • Employed a limited number of normal mode degrees of freedom (< or = 12).

    • Main Results:

    • The normal mode refinement significantly improved the fit to the X-ray diffraction data.
    • The refined F-actin model shows a narrowed nucleotide binding cleft.
    • The DNase I binding loop in the refined model is twisted to a lower radius.

    Conclusions:

    • Normal mode refinement is an effective method for improving F-actin structural models.
    • The refined model's features are consistent with electron microscopy data and other techniques.
    • This approach provides insights into the dynamic rearrangements of actin.