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Quantitation of dopaminergic neurons in 3-dimensional reaggregate tissue culture by computer-assisted image analysis
1Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637, USA.
Journal of Neuroscience Methods
|January 1, 1995
Abstract:
Three-dimensional (3D) reaggregate tissue culture provides a means of quantitatively assessing neuronal cell survival under a variety of experimental conditions. A method is presented for estimation of, and comparison between, the total numbers of cells of a given neurochemical type within individual experimental flasks. The method involves cell counting from random reaggregate sections, determination of sectional volumes, and of 3D reaggregate volumes by a novel image-analysis computer system.