[Differential staining of pancreatic islet A- and B-cells using Rouge's fixative and a modified Gomori method]

Roug's fixative fluid is suggested to fix the pancreas with subsequent staining of the islet A- and B-cells. Rouget's fluid differs from Bouin's fluid in that picric acid is substituted for distilled water, and thus, it is unnecesary to extract the acid from the preparation. The fixation ensures good quality in demonstration specific granulation and preserves it in B-cells. Gormori's modification of the method to stain the slices with aldehyd-fuchsin and subsequent staining with alum carmine and orange G reveals both B- and A-cells. The manipulations presented perfect the quality of histochemical reactions.