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Haemmaccel, a key for lung preservation?

L Spaggiari1, R Alfieri, L Cattelani

  • 1Department of General Thoracic and Vascular Surgery, University of Parma, Italy.

Acta Bio-Medica De L'Ateneo Parmense : Organo Della Societa Di Medicina E Scienze Naturali Di Parma
|January 1, 1994
PubMed
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Haemmaccel (HM), a low-potassium solution, demonstrated superior preservation of isolated lung cells compared to Belzer solution (UWS) and low-potassium dextran solutions in vitro. This suggests HM

Area of Science:

  • Cell Biology
  • Organ Preservation
  • Biochemistry

Background:

  • Lung preservation solutions aim to minimize cellular damage during ischemia.
  • Extracellular, low-potassium solutions show promise over intracellular ones for organ protection.
  • Haemmaccel (HM) is a plasma expander with potential applications in preservation.

Purpose of the Study:

  • To evaluate the efficacy of Haemmaccel (HM) in preserving isolated fetal human lung fibroblasts (WI-38).
  • To compare HM's preservation capabilities against Belzer solution (UWS) and low-potassium dextran (LPD) solutions.
  • To assess the impact of incubation time and concentration on preservation quality.

Main Methods:

  • WI-38 cells were incubated with HM, UWS, and LPD (2%, 5%) solutions at 10°C for 6 and 16 hours.

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  • Cellular viability was quantified by measuring protein synthesis via 3H-leucine incorporation.
  • Statistical analysis was performed using one-way variance analysis.

    • Main Results:

    • HM significantly outperformed UWS in preserving WI-38 cell viability after 16 hours of cold incubation.
    • HM demonstrated significantly better preservation than both 2% and 5% LPD solutions at both 6 and 16 hours.
    • These in vitro findings indicate lower toxicity of low-potassium solutions compared to intracellular solutions.

    Conclusions:

    • Low-potassium solutions, particularly Haemmaccel (HM), offer superior preservation for isolated lung cells compared to intracellular solutions.
    • The colloidal substance polygelin in HM may contribute to its enhanced preservation efficacy over LPD solutions.
    • HM represents a promising alternative for lung preservation, warranting further investigation.