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[Directed cleavage of the 16S rRNA molecule at a single internucleotide bond]
Biokhimiia (Moscow, Russia)
|February 1, 1995
Abstract:
Cleavage of 16S ribosomal RNA (rRNA) from E. coli "hammerhead" type ribozymes as well as by RNAase iI in the presence of "hymeric" (2'-deoxy-F-thymidine containing) oligonucleotides has been studied. The conditions for the cleavage of a desired single internucleotide bond have been found for a large molecule with a very complicated secondary and three-dimensional structure.