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Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:

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Updated: Jan 14, 2026

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Published on: April 3, 2014

Test System for Studying Biotin Transport upon SLC5A6 Gene Inactivation.

A Yu Rudenko^1,2, P A Zotova^1,3, O A Averina¹

¹Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119192 Russia.

|October 22, 2025

Summary

This summary is machine-generated.

Researchers developed a new test system to study biotin transport. Biotin and a derivative can enter cells without the SLC5A6 transporter, suggesting alternative pathways exist.

Keywords:

SLC5A6 biotin biotin derivatives biotin transport cell membrane hSMVT test system

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Published on: December 24, 2016

Area of Science:

Cell biology
Genetics
Biochemistry

Background:

The SLC5A6 gene encodes the sodium-dependent multivitamin transporter (hSMVT), crucial for biotin uptake.
Inactivating SLC5A6 impairs biotin transport, necessitating methods to study alternative pathways.
Understanding vitamin transport is vital for treating genetic disorders affecting nutrient uptake.

Purpose of the Study:

To develop a novel test system for investigating biotin transport after SLC5A6 gene inactivation.
To assess biotin and its derivatives' cell membrane penetration independent of SLC5A6.
To explore alternative cellular uptake mechanisms for biotin and its analogs.

Main Methods:

Utilized modified HEK293 cell lines overexpressing BirA* biotin ligase.
Established two cell lines: one with a functional SLC5A6 gene, another with an inactivated SLC5A6 gene.
Investigated the transport of biotin, Bio-1 (biotin conjugate with p-aminophenylalanine), and Bio-2 (biotin methyl ester).

Main Results:

Biotin and Bio-2 (biotin methyl ester) enter cells independently of the SLC5A6 transporter, indicating alternative transport routes.
Bio-1 is internalized exclusively via the hSMVT transporter (encoded by SLC5A6).
The developed test system successfully differentiates transport pathways.

Conclusions:

Identified alternative cellular uptake pathways for biotin and its methyl ester.
Demonstrated that Bio-1 relies solely on the hSMVT transporter for cellular entry.
The novel test system provides a valuable tool for studying vitamin transport mechanisms and developing therapeutic strategies for genetic transport defects.