Related Experiment Videos
Knockout mice: how to make them and why. The immunological approach
R Fässler1, K Martin, E Forsberg
1Max Planck Institute for Biochemistry, Martinsried, Germany.
International Archives of Allergy and Immunology
|April 1, 1995
Summary
Transgenic technology allows precise genetic alterations in mice using embryonic stem cells. This review details methods for creating genetically modified mice and highlights their use in immunology research.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- Transgenic technology has rapidly advanced.
- Embryonic stem cells bridge in vitro genetic manipulation and in vivo analysis.
- Systematic creation of genetically altered mouse strains is now possible.
Purpose of the Study:
- To review strategies for altering the genetic makeup of mice.
- To summarize results from genetically altered mice in immunology.
Main Methods:
- Review of established transgenic technologies.
- Analysis of genetic manipulation techniques in mouse models.
- Compilation of data from immunological studies using genetically altered mice.
Main Results:
- Detailed overview of various genetic alteration strategies.
- Examples of insights gained from genetically modified mouse models in immunology.
- Demonstration of the utility of transgenic mice for studying immune system function.
Conclusions:
- Transgenic mouse models are powerful tools for immunological research.
- Advances in stem cell technology have revolutionized the creation of genetically altered mice.
- Genetically engineered mice provide critical in vivo models for understanding complex biological processes.