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Interference in triiodothyronine (T3) analysis on the Immuno 1 Analyzer
1Department of Clinical Biochemistry, University of Toronto, Ontario, Canada.
Clinical Biochemistry
|February 1, 1995
Summary
The Immuno 1 Analyzer may show falsely elevated Triiodothyronine (T3) levels. Ethanol extraction of patient samples is recommended to eliminate this interference in T3 analysis.
Area of Science:
- Clinical Chemistry
- Immunoassay Technology
Background:
- Triiodothyronine (T3) is a crucial thyroid hormone.
- Accurate T3 measurement is vital for diagnosing thyroid disorders.
Purpose of the Study:
- To evaluate potential interference in Triiodothyronine (T3) analysis using the Immuno 1 Analyzer.
- To identify and mitigate causes of interference affecting T3 measurements.
Main Methods:
- Analyzed 686 samples for T3 using the Immuno 1 Analyzer.
- Compared Immuno 1 results with radioimmunoassay (RIA) and Ciba-Corning ACS 180 analyzer.
- Investigated serum binding of fluorescein and alkaline phosphatase as potential interference sources.
Main Results:
- Immuno 1 T3 results showed good correlation with RIA and chemiluminescence immunoassay.
- Eight patient samples yielded anomalously elevated T3 results on the Immuno 1.
- Ethanol extraction followed by reconstitution in zero calibrator effectively eliminated interference.
Conclusions:
- The Immuno 1 assay demonstrated a higher susceptibility to interference compared to other methods.
- Recommend assaying ethanol extracts for unexpectedly high T3 results on the Immuno 1 until the assay is reformulated.