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Direct sequencing of PCR amplified pig PrP genes
1Central Veterinary Laboratory (Weybridge), New Hav, Addlestone, Surrey, UK.
Biochimica Et Biophysica Acta
|April 24, 1995
Abstract:
The protein coding regions of the PrP genes of six pigs were sequenced directly from PCR-amplified genomic DNA. All six sequences were identical. The gene encodes a protein of 257 amino acids and shows an overall similarity of 77 to 88% with the PrP sequences from other mammalian species. The significance of amino acids unique to the pig PrP are discussed.