[Erythropoietin in the era of molecular biology]

Cloning of genes for erythropoietin in the mid-eighties started a new period of research and use of erythropoietin. RIA and ELISA methods of its detection were developed. It became possible to seek possibilities of its therapeutic use. The cell type which is the source of erythropoietin in the organism was assessed. The regulation of the expression of the erythropoietin gene in relation to oxygen tension was investigated. The biochemical mechanism is sought which transmits the oxygen tension to transcription signals and the stability of erythropoietin mRNA. The gene for the erythropoietin receptor was found and it is investigated in relation to the pathogenesis of primary polycythemias.