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Vitamin C and glycohemoglobin
C W Weykamp1, T J Penders, H Baadenhuijsen
1Department of Clinical Chemistry, Queen Beatrix Hospital, Winterswijk, The Netherlands.
Clinical Chemistry
|May 1, 1995
Summary
High-dose vitamin C supplementation in nondiabetics does not affect hemoglobin glycation (GHb) measurements. This study found no interference with common GHb testing methods, ensuring accurate results.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Nutritional Science
Background:
- Glycohemoglobin (GHb) is a key indicator of long-term glycemic control.
- Vitamin C (ascorbic acid) is an antioxidant with potential effects on blood glucose metabolism.
- Previous research has yielded conflicting results regarding vitamin C's impact on GHb levels.
Purpose of the Study:
- To investigate the effect of daily vitamin C supplementation on GHb levels in nondiabetic individuals.
- To determine if vitamin C interferes with common GHb measurement techniques.
- To assess the impact of vitamin C on in vivo hemoglobin glycation.
Main Methods:
- A randomized controlled trial involving 30 nondiabetic volunteers.
- Supplementation with 0, 750, or 1500 mg of vitamin C daily for 12 weeks.
- Measurement of GHb using HPLC, electrophoresis, affinity chromatography, and immunoassay at multiple time points.
Main Results:
- Plasma vitamin C levels increased significantly with supplementation.
- No significant differences in GHb, glucose, or fructosamine concentrations were observed between groups.
- Vitamin C supplementation did not interfere with any of the GHb assay methods used.
Conclusions:
- Daily supplementation with 750 or 1500 mg of vitamin C for 12 weeks does not affect GHb levels in nondiabetics.
- Vitamin C does not interfere with the accuracy of GHb determination by common laboratory methods.
- The study does not support a role for vitamin C in reducing in vivo Hb glycation.