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Changes in connexin expression and distribution during chick lens development

J X Jiang1, T W White, D A Goodenough

  • 1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Developmental Biology
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

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Gap junctions in chick lenses change CO2 sensitivity during development. This shift is not solely due to connexin protein expression but likely involves other cellular factors.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biophysics

Background:

  • Gap junctions, formed by connexin proteins, mediate intercellular communication.
  • In the chick lens, connexins 43, 45.6, and 56 form different gap junctions.
  • Lens fiber gap junctions lose CO2 sensitivity during development, unlike epithelial cells.

Purpose of the Study:

  • To investigate the role of connexin expression in the developmental change of CO2 sensitivity in chick lens gap junctions.
  • To determine if specific connexins (Cx 45.6, Cx 56) correlate with CO2 insensitivity in developing lens fibers.

Main Methods:

  • Immunocytochemistry using specific connexin antibodies.
  • Electron microscopy to localize connexins.
  • Xenopus oocyte expression system to measure connexin channel conductances.

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Main Results:

  • Lens fibers express Cx 45.6 and Cx 56 during development, coinciding with CO2 insensitivity.
  • Cx 45.6 and Cx 56 are found in both fiber-fiber and interepithelial junctions.
  • All three connexins (Cx 43, 45.6, 56) exhibited CO2 sensitivity in the oocyte expression system.

Conclusions:

  • Connexin distribution alone does not explain the developmental change in CO2 sensitivity.
  • Post-translational modifications or other cellular context factors likely regulate connexin function and CO2 sensitivity in vivo.